684 Answered Questions for the topic microbiology
07/22/19
Help with preparing Congo Red TSA?
So I want to make Congo Red TSA. My instructor gave some hints but I still have some questions:
1. I start by making two stock solutions: 20 mg/ml of congo red and coomassie in two different...
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Microbiology
07/22/19
Counter Staining with Safranin?
After Gram Staining, We use counter staining to find out that is there any gram(-) bacteria present on the prepared slide. My question is- **What component in the cell wall of gram(-) bacteria is...
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07/22/19
How can I differentiate between polysaccharide bands and protein bands on SDS-PAGE?
I tried to extract bacterial polysaccharide but after running a SDS-PAGE I couldn't differentiate between the polysaccharide band and protein ones. I face a problem of moving up of the samples from...
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07/22/19
How can E. coli affect C. elegans expression?
Plasmids can be transferred to *E. coli*. These transformed *E. coli* can be fed to *C. elegans* to silence its gene expression by RNAi.
How can *E.coli* release RNAi to *C. elegans*? Even if we...
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07/20/19
Can tardigrades survive being eaten?
Compared to a tardigrade, the cockroach seems fragile.
But can tardigrades survive the acidic environment of being eaten by most animals?
07/19/19
Have there been any positive public health effects due to UV lights?
Occasionally, in hospitals and in eating establishments in the US, they have industrial grade UV lights in sconces attached to the wall (though they seem to be less prominent as the years go by). I...
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Microbiology
07/19/19
How are bioremediation microbes removed from the environment once they perform their function?
In the case of bioaugmentation, microbes are introduced into the environment to clean up and absorb a certain pollutant. My question is how is that microbe then removed from the environment once it...
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Microbiology Organic Chemistry
07/19/19
gastric juice have a ph of 1 or 2. this would indicate which of the following?
07/19/19
How can fresh cavities form at the margins of sliver amalgam fillings on teeth?
Silver amalgam fillings predominantly contain silver a known bactericidal agent and mercury which a known toxin and has bactericidal property. So how is it that the plaque bacteria survive near the...
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Microbiology Immunology
07/18/19
Can immunity to diseases vary by populations?
A common explanation for the massive population decreases of isolated societies upon contact with Europeans during the Age of Discovery is that the natives lacked immunity to newly introduced...
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Microbiology Health Occupation Science Section
07/18/19
The process of cellular reproduction in bacteria is known as?
Biology (Cells) Microbiology,practice question for a health occupation entrance exam testing.
07/18/19
Fermentation to acetic acid?
How much time is needed for the fermentation to acetic acid production cycle, and the conversion of apple vinegar in a sealed container incubated at 37 °C? Should be in the fully...
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07/17/19
Do disaccharidases require a cofactor or coenzyme to function?
I'm doing research on lactose intolerance and am curious if disaccharidases (enzymes that break down disaccharides) require a cofactor or coenzyme to function? Reviews or references would be...
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07/15/19
Calculation of the bacterial growth rate from a spectrophotomer growth curve?
Typically the microbial growth in liquid cultures is monitored by turbidity. Data is obtained with a spectrophotometer to measure optical density at 600nm. The slope of the bacterial kinetic curve...
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07/15/19
Can a vaccine or antidote be administrated via gases or sprays?
On TV or in movies a gas or spray containing a vaccine/cure/antitoxin is released and everybody is saved. Is this something plausible in real life? Specific examples would be appreciated.
07/15/19
Can we investigate the cell biology of extinct microorganisms?
I know that there are microfossils, but not if we can we know about the organelles, genetics or macromolecules of the cells that left them behind.
Microbiology
07/15/19
Can some prokaryotic cells be larger than eukaryotic cells?
Can the sizes overlap in some bacterial species? My science teacher claims so. My research says the opposite. Can someone confirm?
07/12/19
Does alpha-amylase from different species have different *optimal* conditions?
Do the optimal conditions for the enzyme isoforms differ between species? Specifically, do the optimal pH and temperature for alpha-amylase differ for that enzyme produced by *B. lichiniformis* and...
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07/12/19
Can the RNA in the HIV virus make viral enzymes without entering the nucleus?
If the provirus was not formed yet, can the virus make viral enzymes?
(I know that it already has some, but supposing it doesn't)
Microbiology Microscopy
07/12/19
Can living cells be imaged with electron microscopy?
According to this article from October 2009, http://news.mit.edu/2009/electron-microscope
> "Electron microscopes are the most powerful type of microscope,
> capable of distinguishing even...
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07/11/19
Can bacteria metabolize fatty acids for fuel?
I'm not a microbiologist, but rather a physiologist curious about microbial metabolism. Much like humans bacteria can utilize glucose, but when it comes to long chain, medium chain, or short chain...
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Microbiology Pathology
07/11/19
Difference between protozoa, protists, protoctista?
Are protozoa, protists, protoctista different classes of organisms or simply different names for the same?
07/11/19
Can mutation rate be increased for instance by mutagenesis targeting the DNA polymerase?
I am studying a non-cultivable bacterium living in an insect host, and I would like to generate some random mutants of this bacteria while preserving the insect.
I am wondering if one way to do so...
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Can we digest food as well as herbivores?
I heard some guy say that we can't digest plant food as well as herbivores. Which seems weird to me seeing as how humans can survive without any nutrient deficiencies besides b12 on only plants but...
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