How can E. coli affect C. elegans expression?
Plasmids can be transferred to *E. coli*. These transformed *E. coli* can be fed to *C. elegans* to silence its gene expression by RNAi. How can *E.coli* release RNAi to *C. elegans*? Even if we assume *E. coli* could enter cells of *C. elegans* for being intracellular Bacteria, it is hard to imagine that RNAi will exit *E.coli* directly and enter *C.elegan*'s cells? Normally, no cells or bacteria will release RNA or genomic material outside of their cells?