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76 Answered Questions for the topic molecular biology

Molecular Biology

08/18/20

. Using basic techniques of molecular biology, design an experiment justifying each method adopted to ascertain the genotoxicity of Arsenic in the population across Gujarat.

A recent news report has shown that in Gujarat, there is a high exposure to Arsenic, especially in regions like Kutch. Using basic techniques of molecular biology, design an experiment justifying... more
Molecular Biology Biology Biochemistry

08/02/20

Intracellular signaling pathways that are participating in protein sorting and membrane traffic

Hi!I am studying biotechnology. Now I'm doing a course of Molecular biology of the Cell, and one of my tasks is to write an essay about "Intracellular signaling pathways". This essay topic is very... more
Molecular Biology Biochemistry Diy Biology

07/31/19

Can PCR tubes be made of different materials than plastic?

Does it matter if I replace the PCR tube (usually made of plastic) with another material like aluminum, glass, or something similar?
Molecular Biology Biochemistry Microbiology

07/29/19

Bacterial cell lysis - what solution to use?

I am trying to determine how quickly detergents act on bacterial cells (cell lysis). I would like to compare some detergents at difference concentrations for bacteriolytic activity. I don’t care... more
Molecular Biology Microbiology Cell Biology

07/22/19

How can E. coli affect C. elegans expression?

Plasmids can be transferred to *E. coli*. These transformed *E. coli* can be fed to *C. elegans* to silence its gene expression by RNAi. How can *E.coli* release RNAi to *C. elegans*? Even if we... more
Molecular Biology Biochemistry Neuroscience

07/19/19

Why do neurones use chemical signalling at synaptic junctions?

When a neurone fires, it sends an electrical signal that jumps down the axon via the nodes of Ranvier very rapidly. At a synaptic junction, chemical Brownian diffusion signalling with receptor... more
Molecular Biology Biology Meiosis

07/18/19

Why did Fair Meiosis evolve?

How and why did Fair Meiosis evolve? I can hardly think that it provided a fitness advantage to the individual carrying the mutation. Why would it? Or did it evolve through lineage selection? Or... more
Molecular Biology Biochemistry Cell Biology

07/18/19

Do lysosomes play any role in cell division?

My biology textbook includes a point that lysosomes stimulates cell division without further elaborating. But studying it, I felt eerie about this function of lysosome. Then, I checked on the... more
Molecular Biology Biochemistry Translation

07/18/19

Do two compatible tRNA codons bond together?

Can two tRNA with complementary anti-codons link together? For instance UUU with AAA. If not, why not?
Molecular Biology Biochemistry Proteins

07/18/19

Do chaperone proteins misfold?

If molecular chaperone proteins assist in the folding process of other proteins and misfolded proteins, can chaperone themselves misfold since they are also proteins? What would happen if... more
Molecular Biology Biochemistry Cell Membrane

07/17/19

Why should phospholipid non-polar tails be "protected" in the membrane bilayer?

>lipids are arranged within the membrane with polar head towards the outer side and non polar tails towards inner side, this ensures that the non polar tail is protected from aqueous... more
Molecular Biology Biology Dna

07/15/19

How much does the Hayflick limit/telomere length vary across taxa and within humans?

Since they seem to be quite connected, I'm curious if anyone knows of research comparing the Hayflick limit (and presumably by extension telomere length) between different taxa. I've heard the... more
Molecular Biology Biochemistry Proteins

07/09/19

What is an aromatic cage and what does it do?

Epigenetics, 2. ed, Chapter 3.6:> Similarly, methylated lysine residues embedded in histone tails can be> read by “aromatic cages” present in chromodomains, or similar domains> (e.g., MBT,... more
Molecular Biology Biochemistry Materials

07/05/19

Is there a reliable source for storage and stability of reducing agents like DTT?

Reading the literature on DTT, one is confronted with a confusing mass of papers; some claim that a 1M solution in water is stable, other papers say it is not. I use the reaction with DTNB to show... more
Molecular Biology Biochemistry Rna

07/02/19

What stops messenger RNA from binding to itself?

Since mRNA is single-stranded, and (mostly) floats freely within the cytosol, what stops it from folding onto itself (like DNA) and preventing transcription?
Molecular Biology Biochemistry Ribosome

07/01/19

What exactly happens if during translation, an amino acid is not present?

Lets say that the cell wants to make a particular protein. Transcription of the appropriate gene is done and the mRNA is made. mRNA attaches to the ribosome and the translation is initiated in a... more
Molecular Biology Biochemistry Dna

07/01/19

Why does high pH result in the denaturation of DNA?

In the Southern blot method, for example, a solution of NaOH is used to denature the DNA in the sample. I find this counterintuitive since I expected that $\ ext{Na}^+$ cations would neutralize... more
Molecular Biology Genetics Spliceosome

06/30/19

What are the different ways an exon gets spliced?

Exons are produced by more than one mechanism, e.g. splicing out introns after transcription, if I remember correctly. Please list all mechanisms.
Molecular Biology Biochemistry Evolution

06/28/19

why is AUG the initiation codon?

Is there any reason why AUG is the initiation codon ?Why is there a need for an initiation codon ? Can't translation start with different codons?
Molecular Biology Biology Cell Biology

06/24/19

Transmembrane Protein Problem?

A transmembrane protein has 1000 aa. The 5th aa is found on the external side of the cell membrane. It interacts with the aqueous environment outside the cell. Amino acid 90 is inside the membrane... more
Molecular Biology Biology Biochemistry

06/24/19

Photosynthesis: What Powers the Splitting of Water?

The splitting of water is an endergonic (non-spontaneous) reaction, and thus would require energy (chemical work to be done) in order to happen.In Photosystem II, an enzyme catalyzes this... more
Molecular Biology Biochemistry Cell Biology

06/24/19

How do centrioles auto-locate to opposite sides of cell during mitosis?

I realize that centrioles are made of 9 triplets of microtubulin wound together with a hollow core, and that they are responsible for the configuration of the spindle during mitosis. The spindle... more
Molecular Biology Biochemistry Human Biology

06/21/19

What do sphingolipids do in humans?

I want to know the significance of sphingolipids in human. I have learnt that sphingomyelin is the most significant type of sphingolipid in human. Also that the sphingomyelin serves as a structural... more
Molecular Biology Biochemistry Botany

06/20/19

What is the relationship between the light and dark reactions of photosynthesis?

My main concern is why are the dark reactions (those that produce hexose) needed? As far as I understand, the sole purpose of photosynthesis is to synthesize carbohydrates, which are only used for... more
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