2 Answered Questions for the topic sds page

How can I differentiate between polysaccharide bands and protein bands on SDS-PAGE?

I tried to extract bacterial polysaccharide but after running a SDS-PAGE I couldn't differentiate between the polysaccharide band and protein ones. I face a problem of moving up of the samples from... more

06/13/19

What kind of "size" does SDS-PAGE separate by?

What is the exact property of the protein that affects migration of bands in SDS-PAGE; is it length, volume, or mass?For example, will `GGGGGGGGGG` (glycine 10-mer; mw <600) migrate more or less... more

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