Can mutation rate be increased for instance by mutagenesis targeting the DNA polymerase?
I am studying a non-cultivable bacterium living in an insect host, and I would like to generate some random mutants of this bacteria while preserving the insect. I am wondering if one way to do so might be to use directed mutagenesis directed towards the bacterial polymerase, which would leads it to increase the error rate. - Does any one has already seen a study with this kind of approach? - Does any one knows or guess another way to obtain some random mutants of the bacteria while preserving the host insect?