4 Answered Questions for the topic gel electrophoresis

Gel Electrophoresis Biochemistry Biotechnology Protocol

07/29/19

Appropriate Buffer for electerophoresis of DNA & Protein TBE or TAE?
Which buffer is best for DNA Electrophoresis and which is best for Protein to be have a sharp bond?Considering a higher electrical conductivity compared to TAE & TBE and the generation of less... more
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Gel Electrophoresis Microbiology Proteins Sds Page

07/22/19

How can I differentiate between polysaccharide bands and protein bands on SDS-PAGE?
I tried to extract bacterial polysaccharide but after running a SDS-PAGE I couldn't differentiate between the polysaccharide band and protein ones. I face a problem of moving up of the samples from... more
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Gel Electrophoresis Biochemistry Molecular Biology

05/07/19

When running a gel, what could cause a standard to run "faster" than usual?
We are running a gel on the products of a restriction digest to determine the size of an insert. We know the vector backbone size should be ~2.9kb; however, the standard appears to be running too... more
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Gel Electrophoresis Biochemistry Lab Techniques

03/14/19

Why should I degas my gel solution for polyacrylamide gels?
In protocols for polyacrylamide gel electrophoresis (PAGE) I often see instructions to degas the gel solution by putting it under vacuum for 10-15 minutes before polymerizing the gel. I usually... more
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