Asked • 07/31/19

Can PCR tubes be made of different materials than plastic?

Does it matter if I replace the PCR tube (usually made of plastic) with another material like aluminum, glass, or something similar?

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PCR Tubes. These PCR tubes are made of high-quality virgin polypropylene and feature uniform thin walls for efficient heat transfer.Whether you are in the market for tubes (typically used more often for short-term reactions or storage), plates (typically used more for screening) or both, the integrity of your samples and reactions is paramount. For this reason, your choice of plasticware requires due consideration. Commonly made of polypropylene, polyethylene, or polycarbonate and glass tubes or pipettee have been used.
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08/04/19

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Inactive Tutor answered • 08/05/19

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Yes, it would effect the PCR reaction. PCR is very temperature sensitive. Glass and Aluminum will react different to temperatures than plastic, and your optimal temperatures will not be reached. If you are using qPCR or real-time PCR, the glass and aluminum will also interfere with the light reaction.

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