445 Answered Questions for the topic biochemistry

11/09/15

If you were to use an antibody to identify a N-acetylglucosamine ( GlCNAC) transferase in a cell, what compartment of the cell would you expect to find this enz

If you were to use an antibody to identify a N-acetylglucosamine ( GlCNAC) transferase in a cell, what compartment of the cell would you expect to find this enzyme?WHY do you say this?

11/09/15

You are mapping a particular sequence of DNA that is 7.5 kb in length...

You are mapping a particular sequence of DNA that is 7.5 kb in length . You ‘cut’ it (by incubation) with HindIII and obtain 3 fragments of 1 kb, 2kb, and 3.5 kb You cut it with Bam HI and obtain 2... more

11/03/15

Please help with biochemistry

DNA topoisomerase II unwinds DNA using the free energy derived from ATP hydrolysis to ADP and Pi. To follow continuously the catalytic turnover of DNA topoisomerse II, an enzymatic assay needs to... more

11/01/15

Please help with biochem

The first law of thermodynamics states that the amount of energy in the universe is constant, but that the various forms of energy can be interconverted. Describe four different types of such... more

10/18/15

I need help with biochemistry

The covalent catalysis mechanism of an enzyme depends on a single active site Cys whose pKa is 8. A mutation in a nearby residue alters the microenvironment so that this pK increases to 10. Would... more

10/05/15

Can beta sheets be found on the surface of proteins?

Protein structure

10/05/15

What is the rationale for many large proteins containing multiple copies of a polypeptide subunit?

 It is question about protein 

04/24/15

why glycolysis occurs in cytoplasm not in mitochondria?

Is there any significance of this? why it can be in mitochondria .

01/26/15

Field of View, please help! I am confused!

1. Explain how the depth of field changed the magnification increased.   2. At what magnification do you have the smallest field of view?    3. Was there a difference in the depth of field with... more

01/26/15

What is the advantage of “pulling” a stain rather than just dropping the stain directly onto the organism?

What is the advantage of “pulling” a stain rather than just dropping the stain directly onto the organism?    

01/19/15

what are three main components or regions to define chromosome

How to define chromosome

10/09/14

What is the effect of an enzyme on the equilibrium state of a biochemical reaction? Explain.

What is the effect of an enzyme on the equilibrium state of a biochemical reaction? Explain.

07/23/14

glycolysis

which phosphate containing molecules are utilize in glycolysis

07/21/14

competitve inhibitor

The Y intercept is 0.01 uM/mon the Vmax is

07/21/14

find the concentration

A solution composed of 0.30 M glucose is consider isotonic to Jurkat cells (a T-cell line) at 250.  If you wanted to make isotonic solution with NaCL instead what concentration of NaCL would you need

07/21/14

to find the k value base on delta G

if delta G0 is 1.7 KJ/mole under standard condition what is K value? k=1 K>1, K<1, K=mc^2 or can not be estimated  

01/06/14

Has anyone performed an experiment demonstrating protein synthesis from "scratch" ?

Has anyone performed an experiment demonstrating that protein synthesis can be performed starting from inorganic materials ? The assumption is one could perform a version of the Urey-Miller... more

10/31/13

what an RNA pseudoknot is. Briefly show how are they involved in frame-shifting, IRES recognition and telomerases.

MY QUESTION IR RELATED TO BIOCHEMISTRY AND it WAS DISCUSSED IN THE ARTICLE "Unraveling the structural complexity in a single-stranded RNA tail: implications for efficient ligand binding in the... more

03/12/13

is a hydrophobic core considered secondary structure

I was wondering whether the hydrophobic core is considered secondary structure? such as alpha helix and beta sheets
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