what is the difference between RT-PCR and PCR?

Question

in terms of things like monitoring gene editing, what are the benefits of using one over the other? how is rt-pcr more beneficial than regular pcr?

Answer 1

Reverse transcription and polymerase chain reaction (PCR) Although they are both molecular biology techniques for amplifying genetic material, polymerase chain reaction (RT-PCR) has different uses. Whereas RT-PCR is specifically made for amplifying RNA—typically cDNA that has been synthesized from mRNA—PCR amplifies DNA directly.

When it comes to monitoring gene editing, RT-PCR is clearly superior to conventional PCR. Researchers can evaluate alterations in gene expression brought about by gene editing treatments using RT-PCR. Studying mRNA levels, alternative splicing events, and differences in RNA transcripts are areas in which it is especially helpful. RT-PCR is the method of choice for examining the functional impact of gene editing at the transcriptional level because it can analyze RNA.

Answer 2

Depending on the level of basic science you are looking for - many answers will be correct. I aimed for a 1st-year biology student explanation but can always go more in-depth if it does not suffice your needs! cheers!