serial dilutions

Question

1. Devise a serial dilution scheme to prepare 1/10, 1/30 and 1/90 dilutions of a disinfectant.

Answer 1

To prepare 1/10, 1/30, and 1/90 dilutions of a disinfectant, you can follow the steps below:

Start with a known volume of disinfectant solution.

Let's assume you have 10mL of the disinfectant.

For the 1/10 dilution:

Take 1mL of the disinfectant solutions and add it to 9 mL of a diluent (such as water or a neutral buffer).

Mix thoroughly.

This will result in a 1/10 dilution, where the final volume is 1omL (1mL disinfectant + 9 mL diluent).

For the 1/30 dilution:

Take 1 mL from the 1/10 dilution prepared in step 2 and add it to 2 mL of diluent.

Mix thoroughly.

This will result in a 1/30 dilution, where the final volume is 3 mL ( 1mL from the 1/10 dilution + 2 mL diluent).

For the 1/90 dilution:

Take 1 mL from the 1/30 dilution prepared in step 3 and add it to 2 mL of a diluent.

Mix thoroughly.

This will result in a 1/90 dilution, where the final volume is 3 mL (1mL from the 1/30 dilution + 2 mL diluent).

Make sure to mix each dilution thoroughly to ensure proper mixing and dilution of the disinfectant,