cell biology question

Question

1. Propose how you would experimentally determine which X, Y, and Z yeast strains correspond with which UCC strains (names and genotypes given at the bottom end) if you had access to the following: 
2. The data on the bottom(table)
3.  X, Y, and Z cultures 
4.  YPD plates (contain all nutrients yeast need to grow) 
5.  -Leu -Trp plates (contain all nutrients except leucine and tryptophan) 
6.  23°C incubator 
7.  37°C incubator 

	Growth at
	23°C	37°C
X	+	-
Y	+	-
Z	+	+

The UCC strains:

name genotype

1. UCC2332 MATalpha Leu- Trp+ CDC13
2. UCC2747 MATalpha Leu+ Trp- cdc13-1
3. UCC2356 MATalpha Leu- Trp+ cdc13-

PLEASE INDICATE STEP WISE EXPERIMENT I WOULD DO! THANK YPOU FOR THE HELP

Answer 1

1. Preparation of Media and Cultures:
2. Prepare YPD plates and -Leu -Trp plates.
3. Label three sets of each type of plate for each of the X, Y, and Z cultures.
4. Initial Culture Growth:
5. Inoculate each X, Y, and Z culture onto a YPD plate. This step is to ensure that the cultures are viable and can grow under optimal conditions.
6. Growth on Selective Media:
7. Inoculate each X, Y, and Z culture onto the -Leu -Trp plates. This step is crucial to test for the ability of each strain to grow in the absence of leucine and tryptophan, which will help identify their genotype regarding Leu and Trp auxotrophy.
8. Temperature Sensitivity Test:
9. Place one set of inoculated plates (both YPD and -Leu -Trp plates) in the 23°C incubator and the other set in the 37°C incubator.
10. The purpose here is to determine the temperature sensitivity of each strain, as indicated by the ability (or inability) to grow at these temperatures.
11. Observation and Recording:
12. After an appropriate incubation period (usually 24-48 hours), observe and record the growth patterns of each strain on each type of media at both temperatures.
13. You're looking for growth (+) or no growth (-) on each plate.
14. Analysis:
15. Match the growth patterns of X, Y, and Z strains with the known genotypes of UCC strains based on their growth on selective media and temperature sensitivity.
16. Specifically:
17. UCC2332 (Leu-, Trp+): Expect growth on YPD at 23°C, no growth at 37°C, and growth on -Leu -Trp at 23°C (but no growth at 37°C).
18. UCC2747 (Leu+, Trp-): Expect growth on YPD at both temperatures, no growth on -Leu -Trp at both temperatures.
19. UCC2356 (Leu-, Trp+): Expect growth on YPD and -Leu -Trp at both temperatures.
20. Conclusion:
21. Based on the experimental results, assign each X, Y, and Z strain to its corresponding UCC strain. For instance, if strain X grows on YPD and -Leu -Trp at 23°C but not at 37°C, it would likely correspond to UCC2332.