Dongteng K. answered • 12/01/20
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First of all, it is impossible to determine the yield of this question without first knowing information regarding the starting materials (concentration, mass, etc...)
Secondly, are you trying to figure out how much water you are adding to each dilution? because it appears that your values are given in molars (Moles/Liter).
Javier C.
The Full problem is: A classmate has grown RW10006, a C. elegans strain that expresses a GFP-histone fusion. They used bioinformatics tools to find out the following: 14.8 kD pI = 9.77 hydrophobicity: about ½ of sequence is above zero and ½ of sequence is below zero, alternating all along the entire sequence They use a dounce and the new better buffer to make a 3mL of protein extract from 1.2g of cells. They then perform a Bradford assay on undiluted extract, a 1/5 dilution of the extract, and 1/2 dilution of the 1/5 dilution of the extract. Here are the absorbance measurements: Undiluted extract: 1.7 1/5 dilution of extract: 0.82 ½ dilution of the 1/5 dilution: 0.49 What is the concentration and yield of the extract?12/01/20