In Glycolysis, where is Magnesium used as a cofactor? Why is it used?

Answer 1

Magnesium is used in glycolysis wherever an enzyme is transferring a phosphate group to or from a molecule within the glycolysis cycle. The reason for its uses as a cofactor is to shield the negative charges from the phosphate groups of ATP to allow the enzyme to properly function.

In glycolysis, Magnesium as a cofactor is used in the first, third, and third steps with the enzymes hexokinase, phosphofructokinase, and phosphoglycerate kinase, respectively.