J.R. S. answered 04/04/18
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Ph.D. in Biochemistry--University Professor--Chemistry Tutor
10% (w/v) by definition means 10 grams/100 ml solution.
Since you want 25 ml (and not 100 ml), then ...
10g/100 ml = 0.1 g/ml x 25 ml = 2.5 g needed to make 25 ml of a 10% (w/v) solution.
NOTE: molar mass of Tris (121 g/mol) is not needed for these calculations.