Felix R. answered 11/01/23
PhD in Microbiology and 10+ Years of Teaching Experience
Dear Nikki,
It would be my pleasure to address this question.
To calculate the final cell counts for WBC (white blood cells), RBC (red blood cells), and PLT (platelets), you'll need to average the counts from both sides of the hemacytometer and then adjust for the 1:100 dilution factor.
Let's proceed with calculating each count:
1. WBC Count:
- Side 1: 6 WBCs in 4 WBC squares
- Side 2: 8 WBCs in 4 WBC squares
- Total WBCs counted = 6 (Side 1) + 8 (Side 2) = 14 WBCs
- To account for the 1:100 dilution factor, multiply the total by 100:
14 WBCs x 100 = 1400 WBCs per uL
So, the final WBC count is 1400 cells per uL.
2. RBC Count:
- Side 1: 70 RBCs in all 10 RBC squares
- Side 2: 63 RBCs in 10 RBC squares
- Total RBCs counted = 70 (Side 1) + 63 (Side 2) = 133 RBCs
- To account for the 1:100 dilution factor, multiply the total by 100:
133 RBCs x 100 = 13300 RBCs per uL
So, the final RBC count is 13300 cells per uL.
3. PLT Count:
- Side 1: 252 PLTs in 15 RBC squares
- Side 2: 243 PLTs in 15 RBC squares
- Total PLTs counted = 252 (Side 1) + 243 (Side 2) = 495 PLTs
- To account for the 1:100 dilution factor, multiply the total by 100:
495 PLTs x 100 = 49500 PLTs per uL
So, the final PLT count is 49500 platelets per uL.
Hope this guidance helps.
Regards,
Dr. Rivera-Mariani
Félix E. Rivera-Mariani, PhD