Sean K. answered 10/10/23
Tutor on STEM subjects at all levels
how do i dilute garlic essential oil or like other essential oils with an emulsifier such as polysorbate 80 to get different concentrations of it (20%, 40%, and 60%)?
This isn't exactly Biology, but typically when people use percent solution, they use volumes. In other words, if you want 20% Garlic essential oil solution in 80% Polysorbate solution to make up total volume of 100%. you will add 1 part Garlic essential oil and 4 parts polysorbate 80 solution. That will give you 20% garlic essential oil solution in polysorbate.
If you have to be more precise, the formula people use is
M1*V1 = M2V2
where
M1 = the concentration of your original essential oil solution
V2 = the volume of your original essential oil solution
M2 = the concentration of diluted essential oil solution in polysorbate
V2 = the volume of the diluted essential oil solution in polysorbate.
So if you wanted say 100 ml of the diluted 20% solution of essential garlic oil in polysorbate, then
M1 = original Garlic solution concentration of 100%
V2 = volume of garlic solution to be added to poly sorbate
M2 = diluted garlic oil solution concentration of 20%
v2 = 100 ml of desired diluted solution volume
so
M1 V1 = M2 V2 becomes
100% * V1 = 20% * 100 ml
Solve for V1
v1 = (20% * 100 ml) ÷ (100%)
= 20 ml of original essential oil
Total volume is 100 ml so the volume of polysorbate you need to add is 100-20 = 80 ml