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Question

The dye molecule 7-aminocoumarin has an extinction coefficient of 3,200M-1cm-1 at 432nm. An aqueous sample of 7-aminocoumarin is placed in a cuvette with a 1.00cm path length and the absorbance is found to be 1.86 at 432nm. What is the molarity of 7-aminocoumarin in this sample? Report your answer to 6 decimal places. (The first few should be zeros...)

NOTE: The terms "extinction coefficient" and "molar absorptivity" are interchangeable; both refer to the "epsilon" term in Beer's Law.

Answer 1

Beers law: A = elc

A = absorbance

e = extinction

l= path length

c = concentration

c = A/el = 1.86 / 3200 x1 = 0.000581 M