Cytosine deanimation of DNA

Question

What is the effect of cytosine deamination on the 5' terminal cytosine of the following DNA sequence,

5'- CAAUCUGGCGUGACG - 3' ?

Also how would this cause a smaller protein to be generated and eluted slower in a size exchange chromatography? (also remember the sequence above is of DNA not mRNA.)

Answer 1

Assuming that the sequence in question is part of a protein-coding gene and is depicted in the reading frame starting CAA UCU GGC . . . Deamination of the 5' cytosine will convert the first codon from CAA (encodes the amino acid glutamine) to TAA, a termination codon. The introduction of this termination codon will result in early termination of the protein coding sequence, producing a shorter protein. This protein will move more slowly through a size exclusion column as smaller molecules enter the pores within the SEC matrix.