Catalyzes and the mechanism of action of the enzyme

Question

show the reaction that catalyzes and the mechanism of action of the enzyme glyceraldehyde-3-phosphate dehydrogenase.

What metabolic pathway does this reaction belong to?

Answer 1

This enzyme plays a role in glycolysis, a metabolic pathway in which glucose is broken down yielding 2 pyruvate and 2 ATP per one 1 glucose (under aerobic conditions). Glyceraldehyde-3-phosphate dehydrogenase (GAP dehydrogenase) catalyzes (step 5) the reaction of 2x glyceraldehyde-3-phosphate (GAP)--> into 2x 1,3-bisphosphoglycerate (1,3-BPG), both now 3 carbon structures. This reaction requires 2 NAD+ +P and yields 2 NADH + H+. The mechanism of action of this enzyme is to catalyze oxidation-reduction reactions. The oxidized NAD+ serves as the electron carrier in this redox reaction. This reaction also starts the 3rd and final stage of glycolysis in which 4 ATP are produced.