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Question

Table for the Identification of Bacteria Associated with the Urinary Tract

Unknown # _6__

	Observation	Result	Explanation of Observation/Result	Organism or Organisms Indicated
Gram reaction &	Shape	Purple spheres, in clumps	Gram positive Cocci	because of the thickness of peptoglycan
MacConkey Agar Plate	No Growth	Gram positive	Because of the clear and growth on MacConkey Agar plate
Eosin-Methylene Blue Agar	No Growth
Urea Hydrolysis	Orange liquid in tube
Indole Production			Yellow ring visible after addition of Kovac’s reagent
Phenylalanine Deamination	No green color after addition of Fecl3
Oxidase reaction	No blue color after addition of oxidase reagent
Nitrate Reduction	No red color after adding nitrate reagents and red color after adding Zn
Catalase	No Bubbles after adding H2O2

Answer 1

TestResultExplanationOrganism Indicated
Gram stain	Gram-positive cocci in clusters	Thick peptidoglycan retains crystal violet stain	Staphylococcus spp.
MacConkey Agar	No growth	MacConkey inhibits Gram-positive bacteria	Gram-positive organism
EMB Agar	No growth	EMB selectively inhibits Gram-positive bacteria	Gram-positive organism
Urea Hydrolysis	Negative	Medium remained orange; urease enzyme absent	Rules out urease-positive organisms (e.g., Proteus)
Indole Production	Negative	No yellow/red layer after Kovac's reagent	Organism does not produce indole from tryptophan
Phenylalanine Deamination	Negative	No green color after FeCl₃	Phenylalanine deaminase absent
Oxidase Reaction	Negative	No blue/purple color developed	Cytochrome c oxidase absent
Nitrate Reduction	Negative	No red color after reagents or zinc	Nitrate was not reduced
Catalase	Negative*	No bubbles after H₂O₂	Catalase enzyme absent