J.R. S. answered 09/21/20
Ph.D. in Biochemistry--University Professor--Chemistry Tutor
It wouldn't give you a different answer if you did it correctly. We always assume 100 g because the definition of PERCENT is parts per 100, not parts per 200 or parts per 700. It just makes our calculations much easier.
If we had, let's say, 23% C (carbon).
If we assume 100 grams of substance, we would have 23 g of C, or 0.23 g of C per g of substance
If we assume 200 grams of substance, we would have 46 g of C, or 0.23 g of C per g of substance.
No difference on a per gram of substance basis.