create a dominant repressor of INO1 and other inositol regulatory genes?

Question

Describe a mutation to the Opi1 transcription factor or another gene that will create a dominant repressor of INO1 and other inositol regulatory genes? How would you test the resulting mutant to see if mutation caused the effects on Opi1 function that you intend?

Answer 1

Hi Mat,

The key to this question is to write out what you know about the regulation of inositol regulatory genes. From there you should be able to imagine a way to crate a dominant repressor of those genes.

If you want help understanding the concepts, shoot me a message.