Techniques used for gene related methods

Question

2. This technique can be used to determine whether or not a particular gene is transcribed in a specific tissue or cell type?

Southern/ RNA-seq/ Western/ In situ immunofluorescence/ Expression library screening/ colony colonization/ or Mass Spec and Database search

3. This technique can be used to identify the gene that codes for a purified protein?

Southern/ RNA-seq/ Western/ In situ immunofluorescence/ Expression library screening/ colony colonization/ or Mass Spec and Database search

4. This technique uses DNA probes to screen a bacterial library?

Southern/ RNA-seq/ Western/ In situ immunofluorescence/ Expression library screening/ colony colonization/ or Mass Spec and Database search

5. Arrange the following events in chronological order, beginning with the earliest:

1 2 3 4 5  migration of an mRNA molecule into the cytoplasm

1 2 3 4 5  polypeptide synthesis

1 2 3 4 5  degradation of an mRNA

1 2 3 4 5  splicing of an RNA molecule

1 2 3 4 5  transcription of a gene

Answer 1

2. This technique can be used to determine whether or not a particular gene is transcribed in a specific tissue or cell type?

Southern/ RNA-seq/ Western/ In situ immunofluorescence/ Expression library screening/ colony colonization/ or Mass Spec and Database search

3. This technique can be used to identify the gene that codes for a purified protein?

Southern/ RNA-seq/ Western/ In situ immunofluorescence/ Expression library screening/ colony colonization/ or Mass Spec and Database search

4. This technique uses DNA probes to screen a bacterial library?

Southern/ RNA-seq/ Western/ In situ immunofluorescence/ Expression library screening/ colony colonization/ or Mass Spec and Database search

5. Arrange the following events in chronological order, beginning with the earliest:

1 2 3 4 5  migration of an mRNA molecule into the cytoplasm

1 2 3 4 5  polypeptide synthesis

1 2 3 4 5  degradation of an mRNA

1 2 3 4 5  splicing of an RNA molecule

1 2 3 4 5  transcription of a gene