i need help to draw the graph and explanation

Question

1.Give the report a descriptive and complete title. 2. State the purpose of running the peroxidase reaction at different pH levels. 3. Submit a bar graph constructed on MS Excel showing the effect of pH on the reaction rate of peroxidase from the data obtained in the laboratory. The bar graph must be labelled properly, with a descriptive title, x-axis title with units, y-axis title with units. 3. In essay format, discuss the effect of pH on peroxidase’s ability to catalyze the reaction as evidenced by the results obtained in the lab. Report must be typed. a. The discussion shall include a quantitative analysis of the data and address these questions: • What assumptions did you make when analyzing and interpreting the m slope values obtained in the lab and used to construct the bar graphs? • Were the results consistent with what is known about how pH affects protein structure and why peroxidase structure is important to its function? • Was your hypothesis regarding the effect of pH falsified or supported? • Given the results you obtained, what do you think the results would look like if 1) more pH levels were tested (rather than just four) 2) peroxidase was extracted from potatoes instead of turnips • Given the results you obtained, how can you improve this experiment to obtain additional relevant data? b. The discussion must include at least one properly formatted citation using published sources. The Council of Science Editors (CSE) provides a variety of ways to cite your references. In the citation-name system, number your sources alphabetically by each author's last name in the reference list at the end of your paper. In the sentences of your paper, cite these sources using the number from the reference list. This means that the in-text citation 1 or (1) refers to the first source in your alphabetical list. Format for books citations: List all authors by last name and initials, separated by commas if there are more than two authors. Put an "and" before the last author in the list. Then put the year of publication, the title of the book (in italics if possible), the publisher, the city, and the number of pages in the book. Format for scientific journal articles citations: List the author(s) of the article using the same format given above for books, then give the year, the title of the article or chapter (no quotes, italics or underlines), then the title of the journal or magazine (in italics if possible), the volume number of the journal (do not use the publication date), and page numbers where the article can be found.

Answer 1

Hi Nahida S.

You possibly have already turned this lab report in, but for future reference: just Google something like "pH and peroxidase activity" and take a look at typical graphings of exactly this type of data. Probably in order to train you in some of the features on Excel, you are asked to make a bar graph, as opposed to a scatter plot. In any event, your pH values go in one spreadsheet column, and the corresponding activity data go in the column adjacent on the right. Then Insert a Chart, select the data block as the range, and set the Chart characteristics as you will. (chart title, axis titles, axis units, etc.). Note that Chart and Graph are terms interchangably used on various graphing spreadsheet programs. And in general, you click on a particular item on the chart (an axis, for example) to open the menus for its various properties.

The results you find on your search as suggested above should help you frame your explanation. Perhaps you could include your thoughts on why an enzyme such as that peroxidase might evolve with the activity profile vs. pH that it has. Is there a benefit to the organism for it to be that sensitive, but not more sensitive or less sensitive? These are the searching sorts of questions which will drive you into research territory, perhaps, someday!

-- Cheers, -- Mr. d.