Which of the following are newly acquired characteristics of E. coli cells successfully transformed with pGLO? select all that apply

Question

a.arabinose production

b.green fluorescence under UV light

c.ampicillin resistance

d.lactose fermentation

Answer 1

The pGLO plasmid has a gene for a protein that confers ampicillin resistance and a gene for green florescent protein (GFP), which is under an arabinose-sensitive promoter. It also has genes for regulatory proteins for the promoter. Cells that receive the plasmid by transformation will contain both the GFP gene and the ampicillin resistance gene. Your answers are b and c. (GFP results in green glowing.)