
Suzanne O. answered 11/30/19
International Experience and Multiple State Certifications
Hi InternetUser 1! Let's break it down into steps to see what we can come up with:
Instructions: For this assignment, you will replicate DNA to make a complementary strand of DNA. Then transcribe the complementary DNA sequence into mRNA. Next, translate the mRNA molecule into the codes for specific amino acids using the amino acid chart on the last page. Lastly, address the reflection questions.
DNA Replication:
Replicate the following strand of DNA:
Original DNA: A T G A A C CA T T C A G T A T G G
Complementary DNA:
Remember that the complimentary base pairs in DNA are Adenine-Thymine and Guanine-Cytosine, or A-T and G-C. This means that if the original sequence is:
A T G A A C C A T T C A G T A T G G
Then the compliment is (replace with the opposite base pairs):
T A C T T G G T A A G T C A T A C C
Transcription:
Transcribe the DNA to make an mRNA molecule
COMPLIMENTARY DNA:
mRNA Molecule:
So if our complimentary DNA (antisense stand) is T A C T T G G T A A G T C A T A C C then to transcribe, we basically create another complimentary strand, but for mRNA, we use Uracil instead of Thymine:
A U G A A C C A U U C A G U A U G G
Translation:
Translate the mRNA into the corresponding amino acids.
mRNA MOLECULE:
AMINO ACID:
Using the DNA sequence provided, determine the amino acids.
Next, take each codon (3-base set) to the table and record the corresponding protein:
A U G = Methionine (start)
A A C = Asparagine
C A U = Histidine
U C A = Serine
G U A = Valine
U G G = Tryptophan
ORIGINAL DNA: A T G G G T C T A G C G A A A G A T
Complementary DNA:
mRNA DNA Molecule:
Amino Acid:
And we do it all again:
- A T G G G T C T A G C G A A A G A T = original DNA
- T A C C C A G A T C G C T T T C T A = complementry DNA
- A U G G G U C U A G C G A A A G A U = mRNA
- A U G = Methionine (start)
- G G U = Glysine
- C U A = Leucine
- G C G = Alanine
- A A A = Lysine
- G A U = Aspartate
Don't forget to address the reflection questions!