What are the effect of microtubule or microfilament inhibition on yeast expression profile?

Question

I was wondering whether anyone has looked at what are the expression changes in yeast when the microtubule or the microfilament polymerization is inhibited? Have there been whole-genome studies?

Answer 1

Inhibiting microtubules or microfilaments in yeast can have various effects on the expression profile of the organism, such as influencing the maintenance of cell shape, intracellular transport, and cell division. Some potential effects of inhibiting microtubules or microfilaments on yeast expression profile are:

1. Cell morphology changes: Microtubules and microfilaments are involved in maintaining the structural integrity of the cell. Inhibition of these cytoskeletal elements can lead to changes in cell shape and overall morphology.
2. Impaired cell division: Microtubules are critical for spindle formation during mitosis, while microfilaments are involved in cytokinesis. Inhibition of either component can result in defects in cell division, leading to changes in the expression of genes involved in cell cycle regulation.
3. Altered intracellular transport: Microtubules and microfilaments serve as tracks for intracellular transport of vesicles, organelles, and macromolecules. Inhibition of these cytoskeletal elements can disrupt normal transport processes, affecting the localization and expression of various genes involved in cellular transport and signaling.
4. Changes in gene expression related to stress response: Disruption of the cytoskeleton can activate stress response pathways in yeast. This can result in the upregulation or downregulation of genes involved in stress response, including those related to heat shock, oxidative stress, or DNA damage repair.
5. Altered cell signaling and gene regulation: The cytoskeleton is involved in signal transduction pathways and can influence gene expression by regulating transcription factors or nuclear organization. Inhibition of microtubules or microfilaments can affect these signaling pathways, leading to changes in gene expression patterns.

Specific effects of microtubule or microfilament inhibition on yeast expression profile can depend on the experimental conditions, the duration, and extent of inhibition.