Determine the sequence of a peptide consisting of 14 amino acids on the basis of the following data.

Question

1. Determine the sequence of a peptide consisting of 14 amino acids on the basis of the following data.

 Amino acid composition: (4S,2L,F,G,I,K,M,T,W,Y)

 N-terminal analysis: S

 Carboxypeptidase digestion: L

 Trypsin digestion: (3S,2L,F,I,M,T,W) (G,K,S,Y)

 Chymotrypsin digestion: (F,I,S) (G,K,L) (L,S) (M,T) (S,W) (S,Y)

 N-terminal analysis of (F,I,S) peptide: S

 Cyanogen bromide treatment: (2S,F,G,I,K,L,M*,T,Y) (2S,L,W)

 M*, methionine detected a homoserine.

Answer 1

SFISGKMTYSLSWL (14 residues)