A gene that is believed to be active in primary metabolism has been sequenced.

Question

A gene that is believed to be active in primary metabolism has been sequenced. Please replicate the sister strand to the code that has been provided. Then transcribe the sister strand. Please note: no introns have been identified. Finally, translate the transcript. Identify the beginning and end of each molecule using the amino and carboxyal groups or the 5', 3' carbon identifications (8pt).


5’-T T A A T G C C T T A A T G G C G G C A A C A A G T G A T G G A C T T-3’

Answer 1

There is AUG in transcribed sister strand, that is methionine and translation start site.

 

Stop codons are UAA, UAG and UGA, considering AUG as start, there are no stop codons in that frame and I do not see any splice consensus  site either.

 

You can look at codon table and translate the polypeptide to write the protein sequence.

 

Hope this helps

Answer 2

Sister strand is:

 

3' A A T T A C G G A A T T A C C G C C G T T G T T C A C T A C C T G A A 5'

 

Transcribed sister gene:

 

5' U U A A U G C C U U A A U G G C G G C A A C A A G U G A U G G A C U U 3'

 

There may be some mistakes.