The molar extinction coefficient of (AMP) is 15,400 𝐿.𝑚𝑜𝑙−.𝑐𝑚 In found the absorbance of a sample of AMP to be 0.63. If length of the cuvette used was 10mm, calculate the concentration of AMP.

Answer 1

Using Beer-Lambert Law: A = ecl

A = absorbance = 0.63

e = extinction coefficient = 15,400 L/mol*cm

c = concentration = ?

l = cell length in cm = 10 mm x 1 cm/10 mm = 1 cm

Solving for c (concentration) = A/el = 0.63/(15,400)(1) = 4.1x10^-5 M