J.R. S. answered 03/14/18
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Ph.D. in Biochemistry--University Professor--Chemistry Tutor
10% w/v solution means 10 g of solute per 100 mls of solution.
To prepare 250 mls, you will need 2.5 x that amount, i.e. 10g/100 ml x 250 ml = 25 grams
Note: the molar mass of Tris does not enter into these calculations because they do not ask for molarity.