Bruno B.

asked • 09/25/17

Question involving enzyme kinetics

Under conditions of saturating amounts of substrate the rate of formation of product is 50 nanomoles per ml of reaction volume every minute. [Hint: what do the words saturating amounts of substrate imply?] The reaction contains 10 nanomole enzyme per ml of reaction volume. The KM of the enzyme is 300 microM determine : a) the concentration of enzyme in microM? b) the Vmax of the reaction in units of microM/s c) The maximum number of molecules of product catalysed by each molecule of enzyme in units of s-1 d) The catalytic efficiency of the enzyme?

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