9 Answered Questions for the topic Enzyme Kinetics

12/25/21

What do you think if, there are two tubes containing the enzyme and substrate (A and B), tube A is the control. and if we double the enzyme levels in tube B; how about Km and Vmax in tube B ?

(whether constant or increasing, I intentionally didn't include the amount of enzyme levels.)
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Enzyme Kinetics Biochemistry Kinetics Enzymes

07/08/19

Why are enzyme-catalysed reactions slower at lower substrate concentration?
Suppose I'm using 200 nmoles of enzyme and 2 mmoles of substrate. The enzyme should be saturated but if I use 50 mmoles of substrate, the reaction will be faster. Why?I just can't get it! Even at... more
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Enzyme Kinetics Biochemistry

06/04/19

Why is competitive inhibition reversible?
My Biochemistry book mentions that 'competitive inhibition' is a reversible form of inhibition.<Br> But given that the inhibitor is blocking the active site and prevents an enzyme-substrate... more
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Enzyme Kinetics Equilibrium

12/29/17

Why is the Kd equation Kd=Koff/Kon instead of K=Kon/Koff?
Lets say a general equation for enzymes is E+S<-->ES<-->E+P, and an expression for the Kd of the enzyme is Kd=[E]+[S]/[ES]. Most K expressions are [Products]/[Reactants], right? In the... more
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Enzyme Kinetics Bio Chemistry

09/26/17

Regarding enzyme kinetics
The KI of a competitive inhibitor is 600 nM. If the KM of the un-inhibited enzyme is 80 nM,  a. What is the KM when 400 nM of inhibitor is present?b. What concentration of inhibitor is needed to... more
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Enzyme Kinetics Biochemistry

09/26/17

Regarding enzyme kinetics
Expressed as a multiple of its KM, how much substrate is required in order for an enzyme to be 50 % saturated? (i.e. what is [S]/KM?). And 98 % saturated?
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Enzyme Kinetics Biochemistry

09/26/17

Regarding enzyme kinetics
According to the Michaelis Menten equation, at what percentage of its maximum rate is an enzyme operating when [S] = ½ KM?
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Enzyme Kinetics Biochemistry

09/26/17

Regarding enzyme kinetics
 1. The KM of an enzyme/substrate combination is 125 mM, with a VMAX of 20 μM.s-1.  The concentration of enzyme is 45 nM. a. At what rate is product being formed when the concentration of substrate... more
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Enzyme Kinetics Biochemistry

09/25/17

Question involving enzyme kinetics
Under conditions of saturating amounts of substrate the rate of formation of product is 50 nanomoles per ml of reaction volume every minute. [Hint: what do the words saturating amounts of substrate... more
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