Describe the three steps of PCR and give the function of the Taq polymerase and the primers

Question

Describe the three steps of PCR and give the function of the Taq polymerase and the primers

Answer 1

Step on e: is denaturation, template  DNA denatures at high heat and two strands are separated.

 

Step two: Primers anneal to single stranded DNA at the right place(1005% complementary pairing) as well as other places.

 

Step 3: Taq polymerase starts DNA replication/synthesis from annealed primers. Replication is  fastest at accurate pairing.

 

These three steps are repeated for 25-30 cycles or more cycles for genomic DNA, since it is too big and complex.

 

please let me know if you need any further information/explanation.