Enzymes activity

Question

explain the requirement of the NaCl solution in the effect of Temperature on Salivary Amylase Activity experiment

Answer 1

From William Reeves's thesis on "The Effects of Various Cation Concentrations on Salivary Amylase Activity", NaCl is required because

"...chloride is necessary for activation of salivary amylase. Salt free amylase is not active."

https://ecommons.luc.edu/cgi/viewcontent.cgi?referer=https://www.google.com/&httpsredir=1&article=3517&context=luc_theses