Explain how Beer-Lambert law is used in spectroscopy?

Question

How would i use the principle behind Beer Lambert and the equation to explain this?

Answer 1

The BL law is used in different ways in spectroscopy, but probably the most common use is in determining the concentration of an analyte in a solution. The law tells us that the absorbance is equal to the concentration times the path length times the extinction coefficient. Put in terms of an equation, we have...

A = ecl

A = absorbance

e = extinction coefficient

c = concentration

l = path length (cm)

Usually, you can look up the extinction for the substance of interest, and using a path of 1 cm (very common), we measure the absorbance (A). We then calculate the concentration of the substance. If you know the concentration (c), i.e., make a standard, you can then find the extinction coefficient. This is another common use fo the BL law.