Sonia B.

asked • 10/02/17

where do you think the bacterium that produces HindIII is found?

The optimal working temperature for the restriction endonuclease HindIII is 37°C

1 Expert Answer

By:

Sonia B.

Hello
Could you help me with this:
perform eight 10-fold serial dilutions of blue dye with water (10-1, 10-2, 10-3, 10-4,
10-5,10-6, 10-7, 10-8) in one row within the 96 well plate provided. Each well will have a
maximum of 100 μL of fluid.
 
How much dye to I have put in the first one (10-1)?
Report

10/10/17

J.R. S.

tutor
That depends on how much is in the well.  If you have 90 ul in the first well, add 10 ul of dye.  And if each successive well has 90 ul, you can transfer 10 ul from the previous well to that one. But note that after transfer, the previous well will now have 90 ul but the final well will have 100 ul.
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10/10/17

Sonia B.

Thanks :)
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10/11/17

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