Sonia B.

asked • 10/02/17

where do you think the bacterium that produces HindIII is found?

The optimal working temperature for the restriction endonuclease HindIII is 37°C

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J.R. S. answered • 10/02/17

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Ph.D. in Biochemistry--University Professor--Chemistry Tutor
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The bacterium that produces HindIII is Haemophilus influenzae, and this bug is usually found in the nasal passages and in the throat of humans.  Is that what you mean?
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Sonia B.

Hello
Could you help me with this:
perform eight 10-fold serial dilutions of blue dye with water (10-1, 10-2, 10-3, 10-4,
10-5,10-6, 10-7, 10-8) in one row within the 96 well plate provided. Each well will have a
maximum of 100 μL of fluid.
 
How much dye to I have put in the first one (10-1)?
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10/10/17

J.R. S.

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That depends on how much is in the well.  If you have 90 ul in the first well, add 10 ul of dye.  And if each successive well has 90 ul, you can transfer 10 ul from the previous well to that one. But note that after transfer, the previous well will now have 90 ul but the final well will have 100 ul.
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10/10/17

Sonia B.

Thanks :)
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10/11/17

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