can you solve this using the C1V1= C2V2 equation

Question

the volume of the water is 125 ul and the volume and the source of protein is 375 stock and the final volume is 500 uL what is the final protein concentration?

Answer 1

Hi! We need a little bit more information about the units of your stock concentration to determine whether this formula can be used, but it sounds like you are on the right track.

C1V1= C2V2

C1, C2 must be in units of concentration (e.g., uM, ug/mL, etc.)

V1, V2 must in some units of volume (e.g., uL, mL, L, etc.)

Let me know if you can provide some more information and we can work through it together.

Answer 2

You can't use C1V1 = C2V2 the way the question is currently written/asked. What is 375? Is that 375 ul, or 375 mg/ml, or 375 uM, or just what is it? If we knew, then maybe we could use the the relationship of C1V1 = C2V2. For example, let us assume, it is 375 ug/ml, then we can use the formula.

C1 = 375 ug/ml = 375 ug / 1000 ul = 0.375 ug / ul)

V1 = 500 ul - 125 ul = 375 ul of the stock

C2 = ?

V2 = 500 ul

(0.375 ug/ul)(375 ul) = (C2)(500 ul)

C2 = 0.281 ug/ul = 281 ug/ml