J.R. S. answered • 12/25/21
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Doubling the amount of enzyme will have no effect on the Km or Vmax. It will alter the absolute velocity, but not the velocity on a per mg protein basis. If you desire additional, more in depth explanation, let us know in the comment section.
J.R. S.
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The Km is the amount of substrate to 1/2 saturate the enzyme and give 1/2 the Vmax. It will not change with increasing amount of enzyme. If you have sufficient substrate to be at Vmax, doubling enzyme will NOT alter Vmax as this is a function of the enzyme structure, not amount. Yes, more product will be formed per minute (increased velocity) but the Vmax per enzyme will be the same.
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12/25/21
Loi J.
Hmm I'm sorry sir,I think you are wrong. Vmax is the maximum velocity which is means the maximum rate at which a reaction can occur given the amount of enzyme concentration. (According to the direvation of michaelis-menten equation and biocem book, lehninger)
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12/27/21
Loi J.
Why did it happen ? Even though I am assuming, as long as there are more substrates than the total enzyme in the original state, we can still add more enzymes so that it will increase the Vmax and consequently the Km in tube B will decrease, because many substrates at 1/2 Vmax form the enzyme-substrate complex when the amount of the enzyme is doubled 🤔 CMIIW12/25/21