J.R. S. answered 10/14/20
Ph.D. University Professor with 10+ years Tutoring Experience
First of all, I think you meant that you dissolved 325 mg of a solute (not a solvent). Anyway, if you dissolved 325 mg in a total final volume of 25 ml, you will then have 325 mg/25 ml = 13 mg/ml, not 0.013 mg/ml. This would be the value of g/ml, i.e. you would have a concentration of 0.013 g/ml which is = 13 mg/ml

J.R. S.
10/14/20
Vivien C.
Furthermore, If It asks me to dilute my standard solution to make 4 other known concentrations by repeating 1ml samples of each would I take 0.1ml of my stock and fill with 0.9ml distilled water? And to work out the new concentration (C2) of my first dilution I would use 25ml= V1 C1=13mg/ml and V2=0.1ml?10/14/20
Vivien C.
Yes, sorry I meant solute. Thank you. My confusion was because it asked it in mg/l so that would be 13000mg/l then.10/14/20