Calculating mass of BSA

Question

Calculate the amount (mass) of BSA (50mg/ml concentration) needed to make 500 ml of 1mg/ml BSA solution.

Answer 1

Use dimensional anlaysis:

500 ml x 1 mg/ml = 500 mg THIS IS THE MASS NEEDED

Your question asked for the MASS needed. Now, if you want to know the volume of the 50 mg/ml solution that you need, then again, using dimensional analysis we find it is 10 ml....

(x ml)(50 mg/ml) = 500 mg

x = 10 ml

Answer 2

To make 500 ml of 1mg/ml BSA, you need 500 mg of BSA (500 ml * 1mg/ml).

Your original concentration of BSA is 50mg/ml. To find out how much of this you need, you just divide:

500mg/(50mg/ml) = 10 ml.