Appropriate Buffer for electerophoresis of DNA & Protein TBE or TAE?

Question

Which buffer is best for DNA Electrophoresis and which is best for Protein to be have a sharp bond?Considering a higher electrical conductivity compared to TAE & TBE and the generation of less heat. If anyone has a particular formulation (add other materials to improve quality) which is obtained as a result of practical experience. I would be grateful if you send me that.

Answer 1

For nucleic acids either TAE or TBE is appropriate. TBE is generally considered to be the better buffer for nucleic acids, but is harder to make because boric acid is fairly insoluble. 5x TBE solutions that aren't autoclaved tend to precipitate. TAE is generally used for preparative gels and lower resolution electrophoresis, and is easier to make. Further TAE can be made at 50X and diluted down, thus reducing time and storage space. These buffers are often used interchangeably. In the old days of radioactive DNA sequencing we used TBE because it has a better conduction and doesn't tend to overheat as much as TAE.