J.R. S. answered • 11/21/17

Ph.D. in Biochemistry--University Professor--Chemistry Tutor

1) v against [S] plot

This clearly gives the least accurate estimate of the kinetic parameters (Vmax and Km) of an enzyme reaction. The main reason for the inaccuracy stems from the fact that this plot takes the shape of a rectangular hyperbola, and the kinetic parameters have to be estimated from a curve that is non-linear.

J.R. S.

tutor

You can only use y = mx + c (form of a straight line) if the data are linear. In a plot of v versus [S], it is only linear at low [S], and that would not be enough data to provide an estimate of V

_{max}.
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11/22/17

Sd K.

11/22/17