Sd K.

asked • 11/21/17

lineweaver burt plot

which of the thtee methods of analysis do you think gives the least accurate estimate? Give reasons for your answer [10marks] 
1) v against [S] plot
2) lineweaver burk plot
3) computer analysis 

1 Expert Answer


J.R. S. answered • 11/21/17

Ph.D. in Biochemistry--University Professor--Chemistry Tutor

Sd K.

Thanks for the Reply,
i was given y=mx + c


J.R. S.

You can only use y = mx + c (form of a straight line) if the data are linear.  In a plot of v versus [S], it is only linear at low [S], and that would not be enough data to provide an estimate of Vmax.


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