Saman S. answered • 7d
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Dunstan’s test is a chemical test for the presence of cholesterol. It typically involves dissolving the sample in chloroform, then adding concentrated sulfuric acid (H₂SO₄). A positive test is indicated by the appearance of a reddish to bluish-green color at the interface of the two layers.
Now, let’s analyze the two substances you're asking about:
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1. Egg Yolk
Contains cholesterol: Egg yolk naturally contains cholesterol (about 186 mg per yolk on average).
Dunstan’s test result: Yes, egg yolk should test positive in Dunstan’s test after extraction of lipids/cholesterol using a non-polar solvent like chloroform. You cannot directly test raw egg yolk without proper extraction, because the proteins and water content interfere.
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2. Pure Cholesterol
Obviously cholesterol.
Dunstan’s test result: Yes, pure cholesterol will definitely test positive in Dunstan’s test, giving the characteristic color change.
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Summary Table:
Substance Contains Cholesterol? Dunstan’s Test Result Notes
Egg Yolk Yes Positive (after extraction) Must be extracted with chloroform first
Pure Cholesterol Yes (100%) Positive Will show clear color change at acid interface
Saman S.
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