Robert S. answered 03/04/21
PhD in Chemistry with industrial R&D and teaching experience
Hello, Airah,
The 20 ml sample will have the same concentration, e.g., Molar, as you point out. But the number of moles of the buffer is dependent on the volume. Moles = volume x concentration. 20 ml represents 20% of the original number of moles in the 100 ml buffer.
Bob

Robert S.
03/04/21
Airah M.
Thanks a lot sir. My speculation of solving a new mole for 20mL was right03/04/21