Calibration curve

Question

Hi,

I need some help with plotting a calibration curve.

I have done serial dilution and I ended up with concentrations as follows:

13000mg/l , 1300mg/l , 130mg/l and so forth.

Now I am not sure what number to go up in (on the x axis) when it comes to drawing the calibration curve....

Thank you for helping

Answer 1

You will plot the mg/ml on the x axis and the dependent variable (probably absorbance) on the y axis. You will generate a linear regression and get the equation in the form of y = mx + b where you can then solve for x given values of y (absorbance) of an unknown.