In using the benedict's test, how could you determine the amount of sugar in the sample?

Question

Is it possible to determine the amount of sugar using the Benedict's test, and if so, how?

Answer 1

One possibility would be to use spectrophotometry and measure the intensity of the color. You would need to know, or find, the appropriate wavelength, but that should be easy if you do a wavelength scan. You would also have to find a suitable standard for the standard curve. There may be other problems, but this would be a possible way to use Benedicts reagent/test to quantify the amount of sugar in the sample. Of course, this would be apply to reducing sugars. If others are present, they would not be detected.