For the experiment measuring concentration of BSA using spectrophotometry, the blank will include which of the following?

Question

For the experiment measuring concentration of BSA using spectrophotometry, the blank will include which of the following? (can choose multiple answer) 1)bradford reagent 2) BSA stock 3)NaCl 4) cell culture

Answer 1

The blank in a spectrophotometric determination of BSA (bovine serum albumin) should include all EXCEPT the BSA stock. This is the blank for tge standard curve. An additional blank should be run for the unknown where it will contain everything except the cell culture. The answer they are probably looking for would be 1, 3, 4.