The chemical equation for the reaction between HCl and albumin (R-NH2-CH-COOH) in the presence of a buffer can be represented as:
HCl + R-NH2-CH-COOH ⇌ R-NH3+CH-COO− + Cl−
In this equation, the proton from HCl is accepted by the amino group (-NH2) of the albumin molecule, forming an ammonium ion (-NH3+). The resulting chloride ion (Cl-) combines with sodium ions (Na+) from the NaOH solution to form NaCl:
NaOH + HCl → NaCl + H2O
The reaction between NaOH and albumin in the presence of a buffer can be represented as:
NaOH + R-NH3+CH-COO− ⇌ R-NH2-CH-COO− + H2O
In this equation, the hydroxide ion (OH-) from NaOH accepts a proton from the ammonium ion (-NH3+) of the albumin molecule, forming water. The resulting carboxylate ion (-COO-) is the conjugate base of the buffer system.
