
Camila M. answered 12/07/24
Vast Experience Teaching College-Level Biology
Let’s tackle your questions on cell respiration, photosynthesis, and cellular biology step by step!
1. Why are molecules such as NAD and FAD needed in substrate-linked reactions?
NAD (Nicotinamide adenine dinucleotide) and FAD (Flavin adenine dinucleotide) are essential coenzymes that play critical roles in cellular metabolism, particularly in substrate-level phosphorylation and the oxidation-reduction reactions (redox reactions) during cellular respiration. Here’s why they are needed:
- Electron Carriers: Both NAD and FAD function as electron carriers. In metabolic pathways, they accept electrons during oxidation reactions. For example, during glycolysis and the citric acid cycle, NAD and FAD capture electrons from substrates being oxidized.
- Energy Transfer: When NAD and FAD are reduced (i.e., gain electrons), they become NADH and FADH22, which carry high-energy electrons to the electron transport chain (ETC). This transfer is crucial for the subsequent generation of ATP through oxidative phosphorylation.
- Regeneration: By participating in redox reactions, NAD and FAD are regenerated for continued cycles of metabolism, enabling ongoing energy production within the cell.
2. In the electron transport chain, why is it useful that oxygen acts as the final electron acceptor?
Oxygen serves as the final electron acceptor in the electron transport chain during aerobic respiration, and this role is critical for several reasons:
- Energy Production: Oxygen’s high electronegativity allows it to efficiently accept electrons from the electron transport chain. This process helps maintain a proton gradient across the inner mitochondrial membrane, which is essential for ATP synthesis via ATP synthase.
- Water Formation: When oxygen accepts electrons, it combines with protons (H++ ions) to form water. This not only removes electrons from the chain but also prevents the buildup of electrons, promoting the continuous flow of electrons through the chain.
- Sustaining Cellular Respiration: By acting as the final electron acceptor, oxygen prevents the blockage of the electron transport chain. If oxygen were absent, the chain would become saturated with electrons, halting the entire electron transport process and leading to cell death.
3. How does oxygen prevent blockages in the system?
Oxygen prevents blockages in the electron transport chain in the following ways:
- Continuous Electron Flow: As the ultimate electron acceptor, oxygen facilitates the continual flow of electrons through the chain. This process is crucial for keeping the components of the electron transport chain active and functioning properly.
- Reduction of Noxious Compounds: By accepting electrons, oxygen prevents the accumulation of reactive oxygen species (ROS) that can result from unpaired electrons. This reduces oxidative stress and damage within the cell.
- Enabling ATP Production: The removal of electrons allows the proton gradient to be maintained, which is necessary for ATP production. If oxygen is not present, the gradient cannot sustain itself, leading to a halt in ATP synthesis.

Camila M.
4. What is a self cell and a non-self cell? Self Cell: In the context of the immune system, a "self cell" refers to cells that belong to an individual's own body. These cells carry specific markers (such as major histocompatibility complex, or MHC molecules) that identify them as "self," and they are typically not attacked by the immune system. Non-Self Cell: In contrast, a "non-self cell" refers to cells that originate from outside the individual’s body, such as pathogens (bacteria, viruses) or transplanted tissues. The immune system recognizes these cells as foreign due to different surface markers and mounts an immune response against them. 5. How to identify if a bacterium is heterotrophic or autotrophic? To identify whether a bacterium is heterotrophic or autotrophic, the following methods can be used: Nutritional Requirements: Heterotrophic Bacteria: Require organic compounds for nutrition (carbohydrates, proteins, etc.). They cannot synthesize their own food and rely on other organisms. Checking for growth on media that contains organic nutrients can indicate heterotrophy. Autotrophic Bacteria: Can synthesize their own food from inorganic substances (e.g., carbon dioxide and minerals). They may use light (photoautotrophs) or chemicals (chemoautotrophs) as energy sources. Metabolic Tests: Bacteria can be tested for specific metabolic pathways: Carbon Source Test: If a bacterium can grow on glucose or other organic compounds, it is likely heterotrophic. If it grows on carbon dioxide as the sole carbon source, it is likely autotrophic. Oxygen Requirement: Autotrophs may vary in their oxygen requirements, while many heterotrophs require oxygen (aerobic) but some do not (anaerobic). Biochemical Testing: Assays can determine the presence of specific enzymes that indicate metabolic pathways associated with either heterotrophy or autotrophy. Understanding these concepts in cell respiration, photosynthesis, and cellular biology helps in grasping the mechanics of life at the cellular level. If you have further questions or need clarification on any point, feel free to ask!12/07/24